Name: Virus Packaging
Brand: Tsingke Biotech
SKU: 00330
Availability: InStock
Rating: 5 (1 reviews)

Overview

Viral packaging refers to the process of inserting foreign genes into the viral genome using the virus's natural structure and functions, and then delivering the foreign gene into target cells by infecting them with the virus. This technique is widely used in gene therapy, gene function research, and vaccine development.

Tsingke has established a Biosafety Level 2 (BSL-2, P2) laboratory, providing a solid foundation for the development and production of high-quality viral products. Leveraging Tsingke's whole industry chainadvantages in gene synthesis, we provide efficient and high quality lentivirus (LV), adenovirus(ADV), adeno-associated virus(AAV),and customized pseudovirus packaging services.

Advantages

High Titer

Efficient lentivirus packaging system with titer ≥1 × 108 TU/mL

High Purity

Ultracentrifugation and filtration for in vivo-grade purity

Ultra-Fast

Gene synthesis and virus packaging delivered in as little as 15 days

Service Details

Lentivirus Packaging	Titer	TAT
Over expression Lentivirus	≥1 E+8 TU/mL（Standard） ≥5 E+8 TU/mL ≥1 E+9 TU/mL	15~25 days，including gene synthesis cycles
RNAi Lentivirus（single target）	≥1 E+8 TU/mL ≥1 E+9 TU/mL
RNAi Lentivirus Set（three target）	≥1 E+8 TU/mL
sgRNA Lentivirus（single target）	≥1 E+8 TU/mL ≥1 E+9 TU/mL
sgRNA Lentivirus Set（three target）	≥1 E+8 TU/mL

Adeno-AssociatedVirus(AAV) Packaging	Volume	TAT
Ultra-purified ssAAV packaging (normal scale serotypes)	5E+11 VGS;1E+12 VGS; 2E+12 VGS;5E+12 VGS; 1E+13 VGS;2E+13 VGS; 5E+13 VGS;1E+14 VGS	12~18 business days,excluding gene synthesis cycles
Ultra-purified ssAAV packaging (Low scale serotypes)	2E+12 VGS;5E+12 VGS; 1E+13 VGS;2E+13 VGS; 5E+13 VGS;1E+14 VGS
Ultra-purified scAAV packaging	5E+11 VGS;1E+12 VGS; 2E+12 VGS;5E+12 VGS; 1E+13 VGS;2E+13 VGS; 5E+13 VGS;1E+14 VGS; 2E+14 VGS

Adeno-AssociatedVirus(ADV) Packaging	Volume	TAT
Ultra-purified scADV packaging	1E+10PFU;5E+10PFU;1E+11PFU	55 business days,excluding gene synthesis cycles

Workflow

Gene Synthesis

Plasmid Preparation

Lentivirus Packaging

Centrifugation

Quality Control & Delivery

Case 1

After the lentivirus was diluted to a uniform titre, 10 μL of virus solution was taken separately and diluted to 10⁶-fold according to a 1:10 gradient. 100 μL of 10-fold diluted and 10⁶-fold diluted viruses were taken to infect 293T cells. One field of view was randomly selected and photographed 72 h after infection (magnification 100×).

Figure1.Fluorescence pictures of 293T infected with different viruses

Case 2

The adenovirus provided by Tsingke had a good effect on the infection of different cells.

Adenovirus	ADV-CMV-EGFP
MOI	1000
Cell	231	hBMSCS	U251	MEF	A549	T24
Virus Effect

Case 3

In vitro application of AAV: Different serotypes of AAV viruses from Kinko's have better infection effect on different cells.

Adeno-associated virus	ssAAV-CMV-EGFP-PolyA(AAV6)	scAAV-CMV-EGFP-PolyA(AAVDJ)	scAAV-CMV-EGFP-PolyA (AAVDJ)	scAAV-CMV-EGFP-PolyA (AAVDJ)
MOI	1000000	4000000	4000000	4000000
Cell	231	231	DC2.4	hBMSCs
Virus Effect

FAQ

The target cells can be transduced by AAV, but why is the GFP fluorescence intensity very low?

The GFP fluorescence intensity in target cells depends on several factors, including the number of viral particles transducing the cells, the proliferative state of the cells, the cell type, and the observation time. Generally, the higher the number of viral particles transducing the target cells, and the faster the cell proliferation, the stronger the GFP fluorescence. For cells with faster proliferation, GFP expression typically peaks 48 to 72 hours after viral transduction. For slower proliferating cells, the GFP expression time will be extended.

The transduction efficiency of AAV in target cells is low. How can the virus transduction efficiency be improved?

At Tsingke, we generally improve transduction efficiency by increasing the MOI (multiplicity of infection). Additionally, maintaining the target cells in good growth condition is crucial to ensure normal transduction efficiency.

What is the packaging capacity of AAV viruses?

rAAV can package approximately 3.0 kb of genetic material. However, if you want to add a marker, such as GFP or RFP, the size of the gene that can be inserted will be smaller, around 1.5 kb.

Why do cells not grow or even die after lentiviral infection?

（1）The most likely reason is: When lentivirus is used, an excessive viral dose can severely damage the cell genome and cell membrane. Therefore, it is necessary to determine the optimal MOI (multiplicity of infection) for each cell type, count the cells based on this value, and then use the appropriate lentiviral dose for the target cell infection.

（2）Other possible reasons: Lentiviral infection itself may induce cell differentiation, which affects the cell's proliferative ability.

（3）If the control lentiviral infection produces normal results but the target lentivirus shows abnormal results, it is possible that the gene targeted by the lentivirus is involved in processes such as the cell cycle, cell proliferation, cell metabolism, or cell adhesion. This situation is difficult to avoid but can generally be addressed by reducing the lentiviral dose, using an inducible expression system, or altering culture conditions.

Why does the company validate effective lentivirus, but it shows no expected effect on the target cells

（1）Functional effectiveness means: Validation is carried out on model cells (e.g., 293T, 3T3 cells) to confirm effectiveness.

（2）For overexpression and related lentiviruses, the expression of the promoter varies across different cell types. As a result, the same lentivirus infecting different cells may produce different effects.

（3）For RNA interference lentiviruses, the background expression levels of the target gene and the differences in the intracellular environment across different cell types may lead to different effects from the same lentivirus infecting different cells.