Name: qPCR Probes
Brand: Tsingke Biotech
SKU: 00302
Availability: InStock
Rating: 5 (1 reviews)

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Home » Product & Services » Oligo Synthesis » qPCR Probes

Overview

qPCR Probes are important raw materials in qPCR. This technology has been widely used in scientific research and production practice, especially in medical testing and diagnosis.

Tsingke has a 100,000-level clean room that meets biomedical diagnostic standards, using internationally advanced synthesis technology, high-quality synthesis reagents, advanced automated production equipment, and strict ISO 13485 quality control system. Tsingke provides customized qPCR probes and oligo primers for qPCR experiments and kit, such as: qPCR Probes、MGB Probes、Double-Quenched Probes and Molecular Beacons etc.

Advantages

Customer Trust

Serving over 500 IVD companies

Stable product quality

High-quality synthetic raw materials and technology, the probes have high purity and low background noise

Anti-contamination Procedures

Strictly control contamination from E.coli and human sources to avoid NTC peaks

Service Details

Service Name	Length (nt)	Purification	Price/ Turnaround time	Deliverable	Application
qPCR Probes	15-30	DSL/OPC/PAGE/HPLC/ Dual PAGE & HPLC	Inquire	· Tube or Customized · Lyophilized DNA ·COA Report (electronic)	The most commonly used types of qPCR experiments
MGB Probes	13-25				For qPCR experiments with higher TM
Double-Quenched Probes	15-45				For qPCR experiments with longer probes
Molecular Beacons	25-40				For qPCR experiments with extremely high sensitivity requirements
Other Probes	Customized	Customized			Special application directions

*Note: In addition to the recommended content, Oligo length and purification methods can also be customized.

Common Fluorescent Dyes and Quenchers types

Fluorescent Dyes	Max Abs	Max Em	Quenchers	Quenching range	Quenching Max
FAM	494 nm	518 nm	Dabcyl	380 nm-530 nm	452 nm
TET	521 nm	536 nm	Eclipse	390 nm-625 nm	522 nm
JOE	520 nm	548 nm	MGB	390 nm-625 nm	522 nm
VIC	538 nm	554 nm	TAMRA	470 nm-560 nm	544 nm
HEX	535 nm	556 nm	BHQ1	480 nm-580 nm	534 nm
Quasar 570	547 nm	570 nm	BHQ2	550 nm-650 nm	579 nm
Cy3	552 nm	570 nm	BHQ3	620 nm-730 nm	672 nm
TAMRA	565 nm	580 nm
ROX	585 nm	605 nm
Texas Red	595 nm	615 nm
Alexa Fluor 633	632 nm	647 nm
Cy5	643 nm	667 nm
Quasar 670	647 nm	667 nm
Cy5.5	684 nm	710 nm
Cy7	750 nm	773 nm

Workflow

high-throughput synthesizer from pmol to mmol levels

Synthesis

high-throughput synthesizer from pmol to mmol levels

waters 2695 & Waters 2767 automatic purification and PAGE purification

Purification

waters 2695 & Waters 2767 automatic purification and PAGE purification

all MASS quality inspection, CE and other additional quality inspections

automatic dispensing instrument for accurate dispensing

Distribution

automatic dispensing instrument for accurate dispensing

tube or Customized、Lyophilized DNA、COA Report

Delivery

tube or Customized、Lyophilized DNA、COA Report

Case

Figure 1: qPCR Probe for SNP target gene quantification

Figure 2: MGB Probe for SNP detection

Figure 3: Long-term stability

Figure 4: Short-term stability

Figure 5: Fluorescence modification stability (70 H)

Related Resource

Tsingke Oligo(DNA) Synthesis Order Form

Oligo Synthesis Flyer V1.1.1.240129

FAQ

How to test the purity of primers?

A common method used in laboratories is the PAGE. For electrophoresis, use a polyacrylamide gel with 7 M urea, 20% polyacrylamide gel for primers with less than 12 bases, 16% polyacrylamide gel for primers with 12-60 bases, and 12% polyacrylamide gel for primers with more than 60 bases. Take 0.2-0.5 OD primer, dissolve with urea saturated solution or add urea dry powder into primer solution until saturated, and heat denaturation (95 ℃, 2 min) before loading sample. The purpose of adding urea is to denaturate, and to increase the specific gravity of the sample, which is easy to add the sample. After a certain period of time (about 2-3 hours), strip the polyacrylamide gel and detect the band type with a fluorescent TLC plate under the UV lamp. There is no impurity under the main band, indicating that the purity is good.
(Sometimes due to insufficient denaturation, there may be bands above the main band, which are primer secondary structure bands.)

Why do dissolved primers work fine at first but not after a period of time?

If the water in which you dissolve the primers has a low pH or is contaminated with bacteria or nucleases, the primers will degrade. Inadequate thawing and mixing during use, and uneven liquid may also cause inaccurate amounts of primers. It is recommended to aliquot primers, avoid repeated freezing and thawing, and use 10 mM Tris pH 7.5 buffer to dissolve primers. There is another possibility that there is no problem with the primers, but that the quality of the materials used in PCR, especially the template, is not completely consistent with that used previously.

Will the primers degrade when transported at room temperature?

It will not degrade, and the dry primer can be stably stored at room temperature for at least two weeks. The average transport time is usually 5days, so the primers you receive will not degrade.

How to measure the OD value of primers?

The OD value of the synthetic primer was determined by using an ultraviolet spectrophotometer with a wavelength of 260 nm and a quartz colorimetric cup with an optical path of 1 cm to determine the optical density of the solution. The optical density of the solution should be diluted to 0.2-0.8. After the DNA dry powder is fully oscillated and dissolved with a certain volume of water, the OD value is measured by diluting with 1 mL of water. OD value of mother liquor should be converted according to dilution ratio. For example, to verify whether the amount of 2 OD primer is accurate, the simple practice is to add 1 mL water, thoroughly dissolve and mix, take 100 μL, add 900 μL water, a quartz colorimetric cup with a light diameter of 1 cm, a wavelength of 260 nm, and the absorbed reading at this time is 0.2.

How many primers of OD value should be synthesized?

According to the purpose of the experiment. In general, about 20 bases of primer 2 OD in PCR amplification can do 400 rxns standard PCR reactions of 50 μL system. If it is to do gene splicing or annealing to do the ligation, 1 OD is enough.

**For Research Use Only. Not for use in diagnostic procedures.

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