Name: Custom DNA Oligos
Brand: Tsingke Biotech
SKU: 00304
Availability: InStock
Rating: 5 (1 reviews)

Custom DNA Oligos

Overview

DNA Oligos are short, single-stranded DNA molecules, usually consisting of 20 to 30 base pairs. These DNA oligos play a crucial role in various molecular biology applications, including PCR amplification, gene cloning, sequencing, gene expression analysis, and other molecular biology experiments.

Tsingke’s DNA oligos, synthesized using cutting-edge technology, boast high purity levels with 100% sequence accuracy. Each DNA oligo is meticulously monitored during synthesis and controlled according to Tsingke's stringent quality assurance and quality control standards. By integrating synthetic raw materials, equipment, technology, and services, Tsingke ensures a seamless industrial chain, delivering safe, reliable, and cost-effective customized DNA oligos. These custom DNA oligos cater to the needs of scientific research and R&D production across universities, research institutes, hospitals, government agencies, and pharmaceutical diagnostic companies.

Advantages

Our custom DNA oligos service provides a cost-effective solution for high-quality DNA oligo synthesis with 100% mass spectrometry detection, ensuring the highest precision and reliability. With over 200 available chemical modifications, we offer customizable options for custom modified DNA oligos to meet diverse research needs. Additionally, we ensure flexibility in production scale, from micrograms to kilograms, accommodating projects of any size. Our comprehensive quality control, DNA oligo synthesis, ensures you receive the best results for your specific applications.

Various QC Methods

100% mass spectrometry with a variety of additional detection options

Various Modification Types

Over 200 chemical modifications available for your selection

Large-scale

Flexible synthesis specifications, μg~kg delivery ability

Service Details

For custom DNA oligos, we offered cover a wide range of needs, from common oligos synthesis (5-60 nt) to long oligos synthesis (60+ nt) and modified oligos synthesis (5-120 nt). The synthesis process ensures high purity through options such as DSL, OPC, PAGE, and HPLC. For more specialized requirements, custom DNA oligos are available, with additional modifications and custom lengths. Tsingke also provides custom modified DNA oligo synthesis, offering tailored solutions for unique research and clinical needs. These custom DNA oligos are available with various modifications such as fluorophores, spacers, and modified bases, ensuring that they meet the specific demands of DNA oligo synthesis projects. With guaranteed mass spectrometry detection, these custom DNA oligos are produced with precision and quality for a wide array of applications in molecular biology, genomic research, and drug development.

Service Name	Length (nt)	Purification	Price/ Turnaround time	Deliverable	Application
Common Oligos	5-60	DSL/OPC/PAGE/HPLC	Inquire	· Tube or Customized · Lyophilized DNA · COA Report (electronic)	PCR、DNA Sequencing
Long Oligos	60+	PAGE/HPLC/Dual PAGE & HPLC			NGS, genomic Research
Large-scale Oligos	Customized	Customized			New drug screening, drug production
Modified Oligos	5-120	DSL/OPC/PAGE/HPLC/ Dual PAGE & HPLC			Various molecular biology research

Common modification types

Spacers	Linkers	Fluorophores	Quenchers	Modified Bases	Other Modifications	Degenerate Base
Spacer C3	Acrydite	Alexa 633	BHQ1	2'-F-rA/rC/rG/rU	Cholesteryl	B=G,C,T
Spacer C6	Azide	CY3	BHQ2	2'-O-Methyl A/C/G/U	Ferrocene	D=A,G,T
Spacer C12	Biotin	CY5	BHQ3	inverted dT		H=A,C,T
Spacer 9	Biotin-TEG	CY5.5	Dabcyl	m5C/m5dC		K=G,T
Spacer 12	CHCH	CY7	Eclipse	ddC		M=A,C
Spacer 18	CHO	ET-ROX	MGB	dI		N=A,G,C,T
dSpacer	COOH	ET-TAMRA	XEN	dU		R=A,G
	C6-NH2	FAM		5F-dU		S=G,C
	C7-NH2	HEX		LNA-A/C/G/T		V=A,G,C
	C12-NH2	JOE		m6A/m6dA		W=A,T
	DBCO	PET		Phosphorylation		Y=C,T
	Digoxin	Quasar 570		Phosphorothioate
	Thiol SH	Quasar 670		RNA base
	Thiol SS	ROX
		TAMRA
		TET
		Tsxas Red
		VIC

Workflow

High-throughput synthesizer from pmol to mmol levels

Synthesis

High-throughput synthesizer from pmol to mmol levels

Waters 2695 & Waters 2767 automatic purification and PAGE purification

Purification

Waters 2695 & Waters 2767 automatic purification and PAGE purification

All MASS quality inspection, CE and other additional quality inspections

Automatic dispensing instrument for accurate dispensing

Distribution

Automatic dispensing instrument for accurate dispensing

Tube or Customized、Lyophilized DNA、COA Report

Delivery

Tube or Customized、Lyophilized DNA、COA Report

Case

Figure 1. Coupling efficiency ：99.5%

Figure 2. MS: Molecular weight deviation ≤ 0.05%

FAQ

What customization options are available for custom DNA oligos?

The entire process from synthesis, QC to packaging can be highly customized. Customizable options include, but are not limited to:

Packaging in 1.5 mL, 15 mL, or 50 mL tubes

Capillary electrophoresis analysis

High-performance liquid chromatography (HPLC) analysis

Polyacrylamide gel electrophoresis (PAGE) analysis

Quantitative PCR (qPCR) analysis

Full-wavelength scanning

OD260/280 measurement

Provision of 1 OD sample with each shipment

Specialized consumables

Customized Certificate of Analysis (COA)

Why do long chain primers cost more than short chain primers?

When combining long chain primers, more reagents are required, especially primers longer than 90 base. Due to the increase in cost, the price is higher.

What are the methods of purification?

The common purification methods are DSL, OPC, PAGE,HPLC and Dual PAGE & HPLC.

Why is the yield of modified primers lower and the price higher than that of general primers?

1. Stability and Efficiency: Modified monomers are less stable and have longer coupling times, resulting in lower efficiency and yield.

2. Purification: They require intensive purification methods like PAGE or HPLC, leading to significant material losses.

3. Raw Material Cost: The raw materials for modified primers are much more expensive, often hundreds of times more than those for general primers.

**For Research Use Only. Not for use in diagnostic procedures.

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