Name: Custom DNA Oligos
Brand: Tsingke Biotech
SKU: 00304
Availability: InStock
Rating: 5 (1 reviews)

Custom DNA Oligos

Overview

DNA Oligos are short, single-stranded DNA molecules, usually consisting of 20 to 30 base pairs. These DNA oligos play a crucial role in various molecular biology applications, including PCR amplification, gene cloning, sequencing, gene expression analysis, and other molecular biology experiments.

Tsingke’s DNA oligos, synthesized using cutting-edge technology, boast high purity levels with 100% sequence accuracy. Each DNA oligo is meticulously monitored during synthesis and controlled according to Tsingke's stringent quality assurance and quality control standards. By integrating synthetic raw materials, equipment, technology, and services, Tsingke ensures a seamless industrial chain, delivering safe, reliable, and cost-effective customized DNA oligos. These custom DNA oligos cater to the needs of scientific research and R&D production across universities, research institutes, hospitals, government agencies, and pharmaceutical diagnostic companies.

Advantages

Our custom DNA oligos service provides a cost-effective solution for high-quality DNA oligo synthesis with 100% mass spectrometry detection, ensuring the highest precision and reliability. With over 200 available chemical modifications, we offer customizable options for custom modified DNA oligos to meet diverse research needs. Additionally, we ensure flexibility in production scale, from micrograms to kilograms, accommodating projects of any size. Our comprehensive quality control, DNA oligo synthesis, ensures you receive the best results for your specific applications.


Various QC Methods	Various Modification Types	Large-scale
100% mass spectrometry with a variety of additional detection optio ns	Over 200 chemical modifications available for your selection	Flexible synthesis specifications, μg~kg delivery abili

Service Details

For custom DNA oligos, we offered cover a wide range of needs, from common oligos synthesis (5-60 nt) to long oligos synthesis (60+ nt) and modified oligos synthesis (5-120 nt). The synthesis process ensures high purity through options such as DSL, OPC, PAGE, and HPLC. For more specialized requirements, custom DNA oligos are available, with additional modifications and custom lengths. Tsingke also provides custom modified DNA oligo synthesis, offering tailored solutions for unique research and clinical needs. These custom DNA oligos are available with various modifications such as fluorophores, spacers, and modified bases, ensuring that they meet the specific demands of DNA oligo synthesis projects. With guaranteed mass spectrometry detection, these custom DNA oligos are produced with precision and quality for a wide array of applications in molecular biology, genomic research, and drug development.

Service Name	Length (nt)	Purification	Price/ Turnaround time	Deliverable	Application
Common Oligos	5-60	DSL/OPC/PAGE/HPLC	Inquire	· Tube or Customized · Lyophilized DNA · COA Report (electronic)	PCR、DNA Sequencing
Long Oligos	60+	PAGE/HPLC/Dual PAGE & HPLC			NGS, genomic Research
Large-scale Oligos	Customized	Customized			New drug screening, drug production
Modified Oligos	5-120	DSL/OPC/PAGE/HPLC/ Dual PAGE & HPLC			Various molecular biology research

Common modification types.

Modification List				Degenerate Bases
5' Mod	3' Mod	Double Label Modifications	Internal Modifications	Codes
5'Alexa Flour 350	3'Alexa Flour 350	CY3,BHQ2	(dT-Alexa Flour 350)	B=G,C,T
5'Alexa Flour 405	3'Alexa Flour 405	CY3,MGB	(dT-Alexa Flour 405)	D=A,G,T
5'Alexa Flour 488	3'Alexa Flour 488	CY5,BHQ2	(dT-Alexa Flour 488)	H=A,C,T
5'Alexa Flour 532	3'Alexa Flour 532	CY5,BHQ3	(dT-Alexa Flour 532)	K=G,T
5'Alexa Flour 546	3'Alexa Flour 546	CY5,MGB	(dT-Alexa Flour 546)	M=A,C
5'Alexa Flour 555	3'Alexa Flour 555	CY5.5,BHQ2	(dT-Alexa Flour 555)	N=A,G,C,T
5'Alexa Flour 568	3'Alexa Flour 568	CY7,BHQ2	(dT-Alexa Flour 568)	R=A,G
5'Alexa Flour 594	3'Alexa Flour 594	CY7,BHQ3	(dT-Alexa Flour 594)	S=G,C
5'Alexa Fluor 610	3'Alexa Fluor 610	FAM,BHQ1	(dT-Alexa Fluor 610)	V=A,G,C
5'Alexa Fluor 633	3'Alexa Fluor 633	FAM,BHQ2	(dT-Alexa Fluor 633)	W=A,T
5'Alexa Flour 647	3'Alexa Flour 647	FAM,DAB	(dT-Alexa Flour 647)	Y=C,T
5'Alexa Flour 680	3'Alexa Flour 680	FAM,ECL	(dT-Alexa Flour 680)
5'Alexa Fluor 700	3'Alexa Fluor 700	FAM,MGB	(dT-Alexa Fluor 700)
5'Alexa Fluor 750	3'Alexa Fluor 750	FAM,TAM	(dT-Alexa Fluor 750)
5'ATTO 425	3'ATTO 425	HEX,BHQ1	(dT-ATTO 425)
5'ATTO 550	3'ATTO 550	HEX,BHQ1	(dT-ATTO 550)
5'ATTO 565	3'ATTO 565	HEX,BHQ2	(dT-ATTO 565)
5'ATTO Rho11	3'ATTO Rho11	HEX,BHQ2	(dT-ATTO Rho11)
5'ATTO 594	3'ATTO 594	HEX,ECL	(dT-ATTO 594)
5'ATTO 655	3'ATTO 655	HEX,MGB	(dT-ATTO 655)
5'ATTO 700	3'ATTO 700	HEX,TAM	(dT-ATTO 700)
5'Acrydite	3'Azide(N3)	JOE,BHQ1	(dT-Azide(N3)
5'Azide(N3)	3'AMCA	JOE,BHQ2	(5F-dU)
5'BHQ1	3'BHQ0	JOE,DAB	(2-F-rA)
5'BHQ2	3'BHQ1	JOE,MGB	(2-F-rC)
5'BHQ3	3'BHQ2	JOE,TAM	(2-F-rG)
5'BIO	3'BHQ3	JOE.ECL	(2-F-rU)
5'BIO-TEG	3'BBQ-650	PHO,FAM	(2'-O-Methyl -A)
5'C6-NH2	3'BKHFQ (Double Quenching)	Quasar 670,BHQ3	(2'-O-Methyl -C)
5'C7-NH2	3'BIO	Quasar570,BHQ2	(2'-O-Methyl -G)
5'C12-NH2	3'BIO-TEG	ROX,BHQ2	(2'-O-Methyl -U)
5'CHCH	3'C3-FAM	ROX,ECL	(dT-Digoxin)
5'CHO	3'C6-NH2	ROX,MGB	(dT-DNP)
5'Cholesteryl	3'C7-NH2	TAM,BHQ2	(dT-BHQ1)
5'COOH	3'CHCH	TAM,DAB	(dT-BHQ2)
5'CY3	3'Cholesteryl	TAM,ECL	(dT-BHQ3)
5'CY5	3'CY3	TAM,MGB	(dT-Biotin)
5'CY5-M	3'CY5	TET,BHQ1	(dT-Chromeo 494)
5'CY5.5	3'CY5-M	TET,BHQ2	(dT-CY3)
5'CY7	3'CY5.5	TET,DAB	(dT-CY5)
5'DAB	3'CY7	TET,ECL	(dT-CY5-M)
5'DBCO	3'DAB	TET,MGB	(dT-FAM)
5'DIG	3'DBCO	TET,TAM	(dT-FITC)
5'ET-ROX	3'ddC	TXR,BHQ2	(dT-HEX)
5'ET-TAMRA	3'DIG	TXR,DAB	(dT-Methylene Blue)
5'ET-B (Blue)	3'ECL	TXR,MGB	(dT-Quasar 570)
5'ET-G (Green)	3'Ferrocene	VIC,BHQ1	(dT-Quasar 670)
5'ET-Y (Yellow)	3'HEX	VIC,BHQ2	(dT-Quasar 705)
5'ET-R (Red)	3'InvdT	VIC,DAB	(dT-ROX)
5'ET-P (Purple)	3'Joe	VIC,MGB	(dT-SF670)
5'ET-0 (Orange)	3'MGB	VIC,TAM	(dT-TAM)
5'FAM	3'Methylene Blue		(dT-VIC)
5'FITC	3'PHO		(dT-NH2)
5'Ferrocene	3'QSY7		(HMC)
5'HEX	3'ROX		(Me-dC)
5'HMC	3'RHO 101		(PC Linker）
5'JOE	3'SF670		(C7-NH2)
5'Me-DC	3'-CT+Signal		(dI)
5'Methylene Blue	3'SH		(dU)
5'NED	3'Spacer(C3)		(dSpacer)
5'PHO	3'SS		(Spacer C3)
5'Quasar 570	3'TAM		(Spacer C6)
5'Quasar 670	3'TXR		(Spacer 9)
5'Quasar 705	3'VIC'		(Spacer C12)
5'ROX			(Spacer 18)
5'rApp			(SS)
5'SF670			(TAO)
5'SH			(XEN)
5'SS			-CT+Signal
5'TAM			LNA-A
5'TET			LNA-C
5'TXR			LNA-G
5'triple Biotin			LNA-T
5'VIC			(dC-Alexa Flour 350)
5'YakYel			(dC-Alexa Flour 405)
			(dC-Alexa Flour 488)
			(dC-Alexa Flour 532)
			(dC-Alexa Flour 546)
			(dC-Alexa Flour 555)
	&nbs

Workflow

High-throughput synthesizer from pmol to mmol levels

Synthesis

High-throughput synthesizer from pmol to mmol levels

Waters 2695 & Waters 2767 automatic purification and PAGE purification

Purification

Waters 2695 & Waters 2767 automatic purification and PAGE purification

All MASS quality inspection, CE and other additional quality inspections

Automatic dispensing instrument for accurate dispensing

Distribution

Automatic dispensing instrument for accurate dispensing

Tube or Customized、Lyophilized DNA、COA Report

Delivery

Tube or Customized、Lyophilized DNA、COA Report

Case

Figure 1. Coupling efficiency ：99.5%

Figure 2. MS: Molecular weight deviation ≤ 0.05%

FAQ

What customization options are available for custom DNA oligos?

The entire process from synthesis, QC to packaging can be highly customized. Customizable options include, but are not limited to:

Packaging in 1.5 mL, 15 mL, or 50 mL tubes

Capillary electrophoresis analysis

High-performance liquid chromatography (HPLC) analysis

Polyacrylamide gel electrophoresis (PAGE) analysis

Quantitative PCR (qPCR) analysis

Full-wavelength scanning

OD260/280 measurement

Provision of 1 OD sample with each shipment

Specialized consumables

Customized Certificate of Analysis (COA)

Why do long chain primers cost more than short chain primers?

When combining long chain primers, more reagents are required, especially primers longer than 90 base. Due to the increase in cost, the price is higher.

What are the methods of purification?

The common purification methods are DSL, OPC, PAGE,HPLC and Dual PAGE & HPLC.

Why is the yield of modified primers lower and the price higher than that of general primers?

1. Stability and Efficiency: Modified monomers are less stable and have longer coupling times, resulting in lower efficiency and yield.

2. Purification: They require intensive purification methods like PAGE or HPLC, leading to significant material losses.

3. Raw Material Cost: The raw materials for modified primers are much more expensive, often hundreds of times more than those for general primers.

**For Research Use Only. Not for use in diagnostic procedures.

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