Our library construction services provide high-quality, ready-to-use sgRNA and site-directed mutagenesis libraries for human and mouse genomes. With precise design and expert validation, we ensure high efficiency, reproducibility, and minimal experimental error. Researchers can perform targeted gene knockouts, activation, or saturation mutagenesis to study protein function, regulatory pathways, and genetic interactions. Our libraries accelerate applications such as drug target discovery, synthetic biology, pathway engineering, and disease mechanism research, supporting high-throughput screening and combinatorial studies. Partner with us to streamline your experiments and achieve reliable, genome-wide functional insights.
| Library Construction | Product Name | Turnaround Time | Deliverables |
| sgRNA library | sgRNA library custruction | Inquiry | Virus: 1 mL, >1E+8 TU/mL Plasmid amount per customer request |
| Human Whole-Genome Knockout Library A (all in one) | 1~3 weeks | Virus: 1 mL, >1E+8 TU/mL | |
| Human Whole-Genome Knockout Library B (all in one) | 1~3 weeks | Virus: 1 mL, >1E+8 TU/mL | |
| Human Whole-Genome Activation Library (all in one) | 1~3 weeks | Virus: 1 mL, >1E+8 TU/mL | |
| Human Membrane Protein Activation Library | 1~3 weeks | Virus: 1 mL, >1E+8 TU/mL | |
| Human Ubiquitination-Related Gene Knockout Library (dual-vector) | 1~3 weeks | Virus: 1 mL, >1E+8 TU/mL | |
| Human Epigenetic Knockout Library (all in one) | 1~3 weeks | Virus: 1 mL, >1E+8 TU/mL | |
| Mouse Whole-Genome Knockout Library A (all in one) | 1~3 weeks | Virus: 1 mL, >1E+8 TU/mL | |
| Mouse Whole-Genome Knockout Library B (all in one) | 1~3 weeks | Virus: 1 mL, >1E+8 TU/mL | |
| Mouse Whole-Genome Activation Library (all in one) | 1~3 weeks | Virus: 1 mL, >1E+8 TU/mL | |
| Mouse Metabolic Gene Knockout Library (all in one) | 1~3 weeks | Virus: 1 mL, >1E+8 TU/mL | |
| Site-directed saturation mutagenesis library | Site-directed saturation mutagenesis library | Inquiry | Inquiry |
Key indicators for assessing the quality of a mutation library include:
Diversity of mutations: The mutations in the library should cover a wide range of variations in the target gene, ensuring sufficient representativeness.
Complexity of the library: This refers to the number of mutant variants in the library, ensuring the library size is large enough to cover possible mutation combinations.
Accuracy of mutations: The accuracy of mutations in the library should be verified using high-throughput sequencing techniques (such as NGS) to avoid the introduction of erroneous mutations.
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