Name: PCR Cloning & Subcloning
Brand: Tsingke Biotech
SKU: 00285
Availability: InStock
Rating: 5 (1 reviews)

Overview

PCR (Polymerase Chain Reaction) cloning and subcloning are essential techniques commonly used in molecular biology for amplifying and manipulating DNA fragments.

PCR cloning streamlines the process by using the polymerase chain reaction to amplify a specific DNA fragment of interest from a template DNA, eliminating the need for specific restriction enzyme sites as in traditional methods. Primers are designed based on the sequence of the target gene, enabling direct amplification of the desired DNA fragment.

Subcloning involves transferring a DNA fragment from one vector to another.

PCR cloning and subcloning enable researchers to clone and manipulate DNA fragments efficiently for various applications in gene expression, sequencing, protein production, and functional analysis.

Tsingke offers a one-stop service ranging from gene synthesis and vector construction to PCR cloning and subcloning. With advanced technology and extensive expertise, we ensure high-quality cloning services to support your research needs.

Advantages

Our PCR cloning and subcloning services offer comprehensive solutions for amplifying and manipulating DNA fragments with 100% sequence accuracy, verified through Sanger sequencing and NGS. Whether you're looking for PCR cloning or subcloning, our one-stop service handles it from gene synthesis to vector construction, ensuring seamless cloning and subcloning processes. With advanced technology, we provide customizable options for any site and vector, making our PCR cloning services ideal for gene cloning, PCR-based cloning, and DNA subcloning needs. Trust us for precise and reliable cloning by PCR at every step.

Accuracy

Guaranteed with Sanger sequencing and NGS

No limit

Not limited by restriction enzyme cutting sites

Clonable

Clone target gene fragment at any site in any vector system

Service Details

Tsingke offers comprehensive PCR cloning and subcloning services with a focus on gene cloning and subcloning techniques. For DNA fragments of varying lengths, the turnaround time is flexible, ranging from 7 to 15 business days, depending on the size of the plasmid (up to 7 kb), with custom evaluation available for larger sizes. Deliverables include lyophilized plasmid DNA (1-4 µg/tube) along with comprehensive QC reports like sequencing maps and target sequences. This service supports applications such as gene subcloning, introduction of mutations, gene library construction, DNA labeling, and gene construction for protein expression, it is the top choice for researchers needs.

Length	*Turnaround time (Business Day)	Deliverables
<3 kb	7~10	1 tube of lyophilized plasmid DNA (about 1~4 μg/ tube); Sequencing map (.abl file); Target sequence (.seq file); COA Report(electronic).
3 kb~5 kb	10~12
5 kb~7 kb	12~15
>7 kb	Evaluation

*Note: Get your accurate estimated turnaround time by emailing gene@tsingke.com.cn

FAQ

What do I need to provide for PCR cloning services?

a.Templates： Provide and send us the template plasmid. If the plasmid was constructed by Tsingke and the order was placed within the last year, you do not need to provide it again.
b.Vector details: Choose from our 160+ free vectors or provide your vector.
c.Provide the sequences and resistance information for both template and vector, along with the target sequence and cloning site information.
d.Order Form: Download the "Tsingke Gene Synthesis Order Form" from our website, fill in the relevant fields according to your needs, and send it via email to gene@tsingke.com.cn to place your order. the a.Templates： Provide and send us the template plasmid. If the plasmid was constructed by Tsingke and the order was placed within the last year, you do not need to provide it again.b.Vector details: Choose from our 160+ free vectors or provide your vector.c.Provide the sequences and resistance information for both template and vector, along with the target sequence and cloning site information.d.Order Form: Download the "Tsingke Gene Synthesis Order Form" from our website, fill in the relevant fields according to your needs, and send it via email to gene@tsingke.com.cn to place your order.

Can I provide PCR products directly for PCR cloning?

No, the template sequence needs to be first constructed into a T-vector or another vector. Then, amplify using the plasmid as the template. Alternatively, you can choose our gene synthesis service by simply providing the DNA sequence.