Recombinant Protein Expression Service

Service Name

Service Code

Service Details

Turnaround Time

Escherichia Coli
Expression System

Tsingke-001

Codon Optimization and Gene Synthesis

1~2 weeks

Subcloning

Expression testing and purification
(optimized under various conditions)

2~3 weeks

Shipment of samples

-

Experiment report

Bacillus Subtilis
Expression System

Tsingke-002

Codon Optimization and Gene Synthesis

1~2 weeks

Subcloning

Expression purification testing

2~3 weeks

1 L fermentation purification

1~2 weeks

Shipment of samples

-

Experiment report

Yeast Expression System

Tsingke-003

Codon Optimization and Gene Synthesis

1~2 weeks

Subcloning

Antibiotic screening

1 week

Pilot protein expression evaluation
(2 positive clones will be used to verify the expression, conducting optimization of methanol concentration and induction time.)

1~2 weeks

Purification testing (using the optimal clone under the best expression conditions for a 100 mL expression purification)

1~2 weeks

1 L fermentation purification

1~2 weeks

Shipment of samples

-

Experiment report

Insect Expression System

Tsingke-004

Codon Optimization and Gene Synthesis

1~2 weeks

Subcloning

P1 virus stock generation and expression testing
 (Plasmid transformation, bacterial growth, blue-white screening, plasmid extraction, transfection, P1 expression verification,  SDS-PAGE or Western blot)

2~3 weeks

P2 virus stock generation  and optimization of expression conditions
(P2 virus generation, MOI testing,  SDS-PAGE or Western blot)

2~3 weeks

200 mL expression purification testing and protein sample delivery

1 week

1 L insect cell scale expression and purification

1~2 weeks

Shipment of samples

-

Experiment report

Mammalian
Expression System

Tsingke-005

Codon Optimization and Gene Synthesis

1~2 weeks

Subcloning

80 mL expression testing

1~2 weeks

1 L scale expression and purification

1~2 weeks

Shipment of samples

-

Experiment report

Deliverables

Delivery Standard

Purified protein; QC analysis.

Optional：

Plasmid contain gene of interest; HPLC-SEC; Endotoxin test; MS etc.

Evaluate each order’s SDS-PAGE result