Recombinant Protein Expression Service

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Overview
In response to the growing need for swift and high-throughput protein and antibody research, Tsingke introduced our pioneering Recombinant Protein Expression Service. Tailored to meet the dynamic requirements of the evolving research landscape, particularly amid the rapid advancements in antibody drug development, this service ensures rapid delivery by leveraging a high-throughput transient transfection expression platform.

Tsingke offers a comprehensive one-stop service, that covers gene synthesis, protein structure analysis and design, protein expression, as well as efficient transfection and purification. Committed to guiding researchers at every step, our Recombinant Protein Expression Service aims to maximize the success of research projects, accelerating the journey toward groundbreaking discoveries.
Advantages
Versatile Options
Tailor expression with diverse choices in promoters, vectors, hosts, and fusion tags
High expression level
Utilizing codon optimization along with QC and protein purity, activity, and endotoxin levels testing
High success rate
Successfully expressed over a hundred proteins in mammalian system

Service Details

Service Name

Service Code

Service Details

Turnaround Time

Escherichia Coli
Expression System

Tsingke-001

Codon Optimization and Gene Synthesis

1~2 weeks

Subcloning

Expression testing and purification
(optimized under various conditions)

2~3 weeks

Shipment of samples

-

Experiment report

Bacillus Subtilis
Expression System

Tsingke-002

Codon Optimization and Gene Synthesis

1~2 weeks

Subcloning

Expression purification testing

2~3 weeks

1 L fermentation purification

1~2 weeks

Shipment of samples

-

Experiment report

Yeast Expression System

Tsingke-003

Codon Optimization and Gene Synthesis

1~2 weeks

Subcloning

Antibiotic screening

1 week

Pilot protein expression evaluation
(2 positive clones will be used to verify the expression, conducting optimization of methanol concentration and induction time.)

1~2 weeks

Purification testing (using the optimal clone under the best expression conditions for a 100 mL expression purification)

1~2 weeks

1 L fermentation purification

1~2 weeks

Shipment of samples

-

Experiment report

Insect Expression System


 

 

Tsingke-004


 

 

Codon Optimization and Gene Synthesis

1~2 weeks

Subcloning

P1 virus stock generation and expression testing
 (Plasmid transformation, bacterial growth, blue-white screening, plasmid extraction, transfection, P1 expression verification,  SDS-PAGE or Western blot)

2~3 weeks

P2 virus stock generation  and optimization of expression conditions
(P2 virus generation, MOI testing,  SDS-PAGE or Western blot)

2~3 weeks

200 mL expression purification testing and protein sample delivery

1 week

1 L insect cell scale expression and purification

1~2 weeks

Shipment of samples

-

Experiment report

Mammalian
Expression System

Tsingke-005

Codon Optimization and Gene Synthesis

1~2 weeks

Subcloning

80 mL expression testing

1~2 weeks

1 L scale expression and purification

1~2 weeks

Shipment of samples

-

Experiment report


Deliverables

Delivery Standard

Purified protein; QC analysis.

Optional:

Plasmid contain gene of interest; HPLC-SEC; Endotoxin test; MS etc.

Evaluate each order’s SDS-PAGE result

Workflow
Workflow
Case
Picture 1: Protein expression in Mammalian Cell
Picture 2: ProteinX expression in Escherichia Coli
Related Resource
FAQ
Does Tsingke provide codon optimization? Does optimization have an impact on gene expression?

Tsingke offers free codon optimization.
For the same amino acid in different hosts, the corresponding codons may exhibit varying degrees of preference. Codon optimization involves utilizing preferred codons and avoiding rare codons to optimize the expression of a sequence in a specified host. There is a strong correlation between gene expression levels and codon preference.
Tsingke's unique GeneOptimizer software utilizes a codon optimization algorithm that supports optimization for tens of thousands of hosts and provides customized services. The main parameters for optimization include codon preference, GC content, restriction enzyme sites (deletion), poly structure, sequence repeats, etc.

Which termination codon is preferred in the E. coli expression system? What about in mammalian systems?

In E. coli, TAG is rarely used; TAA is frequently used; TGA is also available.
In mammals, TGA is used more frequently than TAA and TAG.
Relative to the differences between mammals and E. coli, codon usage tables do not differ very much between different mammalian organisms.

**For Research Use Only. Not for use in diagnostic procedures.
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