Site-directed Mutagenesis

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Overview
Site-directed mutagenesis is a molecular biology technique used to precisely introduce precise changes or mutations into a specific DNA sequence. This includes base insertions, deletions, and point mutations. 

Now site-directed mutagenesis has evolved into a powerful tool for enhancing the expression, traits, and characterization of the targeted protein efficiently. Widely employed in the investigation of protein functional site structures, optimization of enzyme activity, understanding of DNA component functions and interactions, site-directed mutagenesis service is also the ideal solution in gene therapy and diverse research fields.

Tsingke provides specialized and cost-effective site-directed mutagenesis services, precisely introducing your requirement mutations for any gene template and any target number of mutations. With expert primer design and advanced mutagenesis methods, we ensure increased experimental efficiency and cost-effectiveness, facilitating your research endeavors seamlessly.

 

Advantages
Our site-directed mutagenesis service delivers unmatched accuracy, supported by Sanger sequencing and NGS. With the flexibility to introduce unlimited mutation sites, you can design mutagenesis primers for any target region with confidence. Our cost-effective approach treats all mutations within a 30-base region as a single mutation, making our service both efficient and economical. Whether you require advanced mutagenesis techniques or expert guidance on mutagenesis primer design, our solutions are tailored to meet your specific research needs.
Accuracy
Guaranteed with Sanger sequencing and NGS
Unlimited Sites
Introduce your mutations at any site
Cost-effective
All mutations found within a 30-base region will be defined as one whole mutation
Service Details
Our site-directed mutagenesis service provides a comprehensive solution, offering expert site-directed mutagenesis primer design and advanced mutagenesis methods. With a swift turnaround time and high-quality deliverables, our mutagenesis services ensure efficient and accurate results. Whether which length you need, our service is tailored to meet your research needs. Contact us to receive an accurate estimated turnaround time and discover how our site-directed mutagenesis service can enhance your projects.

Length

*Turnaround time

(Business Day)

Deliverables

<3 kb

7~10

1 tube of lyophilized plasmid DNA
 (about 1~4 μg/ tube); 

Sequencing map (.abl file);

Target sequence (.seq file);

COA Report(electronic). 

3 kb~5 kb

10~12

5 kb~7 kb

12~15

>7 kb

Evaluation

*Note: Get your accurate estimated turnaround time by emailing gene@tsingke.com.cn
Workflow
oligo design & synthesis
Order Design
oligo design & synthesis
PCR amplification or digestion
Sequence Acquisition
PCR amplification or digestion
linearized the purified DNA fragment with the cut destination vector
Vector Construction
linearized the purified DNA fragment with the cut destination vector
QC for NGS & Sanger sequencing
Quality Control
QC for NGS & Sanger sequencing
ship the plasmid containing your target gene fragment
Delivery
ship the plasmid containing your target gene fragment
Case 1
Figure1.Single Point Mutation

Case 2
Figure2.Double point mutation
Related Resource
FAQ
What do I need to provide for site directed mutagenesis service?
a.DNA template: Please send us your template.
b.Information: The sequence of the template including the target gene and vector information, mutation specifications.
c.Order Form: Download the "Tsingke Gene Synthesis Order Form" from our website, fill in the relevant fields according to your needs, and send it via email to gene@tsingke.com.cn to place your order.
How is the pricing for site-directed mutagenesis determined? Is there a charge for each mutated base?
The pricing for site-directed mutagenesis is evaluated and quoted based on the specific location of the mutation sites and the number of mutations (all mutations within a 30-base region will be considered as one mutation).
**For Research Use Only. Not for use in diagnostic procedures.
Send Your Request
Want to learn more about these services? Speak to our experts and get a free quote.
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