Expert Gene Synthesis Services -Tsingke

Overview

Gene synthesis is a sophisticated biotechnological process that involves the artificial construction of custom DNA sequences based on specified genetic information. Gene synthesis plays a crucial role in various fields such as genetic engineering, synthetic biology, drug development, and protein expression studies.

Tsingke is a premier gene synthesis service provider, offering high-quality services with fast turnaround time. We have established the autonomous research and development, automated self-aggregation TSINGKE Gene Factory synthesis platform, equipped with advanced Tsynth™ synthesis technology. Based on TSINGKE HELIXTECH, we integrate synthetic materials, synthetic equipment, and synthesis processes in the gene synthesis elements, achieving a highly intelligent production system. Continuous research and upgrades to our synthesizers and materials ensure the production of highly accurate oligos, enabling stable and efficient gene synthesis with timely delivery. For quality assurance, all Tsingke-constructed plasmids undergo rigorous NGS and Sanger sequencing validation, ensuring 100% sequence accuracy.

Advantages

Tsynth™ Gene Synthesis sets the benchmark in the field, offering unparalleled speed, precision, and flexibility. As a leader among gene synthesis companies, our advanced technonlgy allows us to efficiently construct even large sequences, plasmids up to 200kb in length. Our gene synthesis service provides access to over 160 commercial vectors at no additional cost, complete with free codon optimization, ensuring your custom gene synthesis projects are both economical and highly effective. With a unique intelligent splitting algorithm, we streamline the design of oligos for each gene, delivering results as fast as 5 days for standard gene synthesis. Whether you need custom plasmid synthesis or comprehensive plasmid preparation, Tsingke offers a superior gene synthesis service tailored to your needs.

Achieved construction of 200kb plasmid

Proficient in synthesizing large sequences

Optional

Choose from 160+ commercial vectors at no extra cost, and enjoy free codon optimization

Fast Turnaround Time

As fast as 5 days for standard gene synthesis

Unique Intelligent Splitting Algorithm

Rapidly and scientifically designs oligos for each gene

Service Details

Tsynth™ Gene Synthesis delivers top-tier gene synthesis services with unmatched speed and precision. Our offerings include highly customizable custom gene synthesis, extensive plasmid synthesis services, and efficient plasmid preparation with low endotoxin levels. We provide rapid turnaround times, with results available in as fast as 5 days, and ensure 100% sequence accuracy through Sanger sequencing and NGS. Whether you need custom plasmid synthesis, DNA fragments, or advanced mutagenesis, Tsingke stands out among gene synthesis companies for its comprehensive, reliable, and rapid solutions.

Standard Gene Synthesis & ProLongGene	- Highly Customized: From simple sequences to complex sequences - Gene Length: Up to 200 kb - Standard Deliver 1~4 μg plasmid - Fast Turnaround: As fast as 5 business days
DNA Fragment	- From 100 bp to 1.2 kb - Deliver 500 ng or 1 μg PCR products - Efficient turnaround time of 2 to 5 business days
Single-stranded DNA (ssDNA)	- From 100 nt to 6000 nt - Deliver lyophilized product powde
Plasmid Preparation	- Research grade - Industrial grade - Endotoxin <0.1 Eu/μg, <0.01 Eu/μg, <0.005 Eu/μg
PCR Cloning & Subcloning	-100% Sequence Accuracy: Guaranteed with Sanger sequencing and NGS -Not limited by restriction enzyme cutting sites -Customizable: Clone target gene fragment at any site in any vector system
Mutagenesis	-100% Sequence Accuracy: Guaranteed with Sanger sequencing and NGS -Unlimited Sites: Introduce your mutations at any site -Cost-effective: All mutations found within a 30-base region will be defined as one whole mutation

FAQ

Does Tsingke provide codon optimization? Does optimization have an impact on gene expression?

Yes, Tsingke offers free codon optimization.

Codon optimization enhances gene expression by using preferred codons and avoiding rare ones for a specified host. This optimization correlates strongly with improved gene expression levels. Tsingke's unique GeneOptimizer software supports optimization for tens of thousands of hosts and offers customized services. Key optimization parameters include codon preference, GC content, restriction enzyme sites (deletion), poly structures, and sequence repeats.

Can you keep my sequence information confidential?

Yes, all customer information will be kept confidential.

Can I send my vectors to you? Are there any requirements for my provided vector?

Yes, you can use your own vector. It is recommended to ship your vector in advance; otherwise, the turnaround time may be extended by 3 business days to verify the vector.

We need you to ship at least 2 μg of the plasmid and provide the full sequence information of the vector. If the full sequence is not available, please provide at least 200 bp upstream and downstream of the cloning site, along with the vector's antibiotic resistance information.

What vector does Tsingke use by default?

By default, we provide pUC57 vectors containing Amp (ampicillin) for free. We also offer a library of over 160 vector options available for free.

What information do I need to prepare a quote?

We need the insert DNA, host species (if codon optimization is needed), and restriction sites at the 5'/3' ends. Specify any internal modifications to avoid and additional elements like the Kozak sequence or stop codon.

How do I place an order?

Complete the gene synthesis Request Forms and email it to info@tsingke.com.cn. For special inquiries or project requirements, please specify them in the email. Our technical support team will promptly provide a detailed quotation.