Oligo Synthesis, Gene Synthesis Solution Provider

Overview

Since the discovery of the double helix structure of DNA by James Watson and Francis Crick in 1953, mankind's exploration of the mysteries of life has entered a brand new era, and the publication of the human genome atlas in 2003 marked the official arrival of the genome sequencing era. In this technological revolution, genome sequencing technology has made continuous breakthroughs, from classic Sanger sequencing to high-throughput sequencing, and each advancement has greatly promoted the development of biology and medicine.

As a leading genome sequencing service provider in the industry, Tsingke gene factory has provided high-quality sequencing services to tens of thousands of clients with our outstanding technical strength and professional service team. Tsingke are equipped with the advanced 3730XL Genetic Analyzer, conducts structural and functional analysis of genomes. Tsingke are realizes the automation and intelligence of the entire process from sample processing to data generation, ensuring accuracy at every step and a sequencing success rate of over 99%. Meanwhile, genetic testing technology not only provides an important tool for scientific research, but also provides solid technical support for our core business - gene synthesis, ensuring the accuracy and reliability of gene synthesis.

Technical Strength

-Sanger Sequencing: Tsingke is equipped with multiple advanced 3730XL Genetic Analyzers, which realize the automation and intelligence of the whole process from sample processing to data generation. This not only dramatically improves sequencing efficiency, but also ensures a high sequencing success rate, providing reliable data support for gene synthesis.

-FastNGS sequencing: For the special needs of small genome sequencing, Tsingke have innovatively introduced FastNGS sequencing technology. This technology not only dramatically increases the sequencing speed, but also significantly improves the accuracy and consistency of data, providing efficient support for gene synthesis.

-2nd and 3rd generation sequencing: With Illumina high-throughput sequencing platform and 3rd generation sequencing platform, Tsingke has rich experience in multi-omics analysis. Whether it is genome, transcriptome, proteome and other multi-omics research, or complex gene synthesis detection, we can provide comprehensive solutions to meet the needs of different customers.

Advantages

High Accuracy

The high accuracy of Sanger sequencing ensures precise genetic testing for applications requiring a high degree of accuracy. Tsingke\\\\\\\\\\\\\\\'s 3730XL Genetic Analyzer automates the entire process.

High throughput

NGS can generate millions of short reads and long sequences in a single run, making it suitable for large-scale genome and transcriptome analyses, and Tsingke\\\\\\\\\\\\\\\'s Illumina platform meets the needs of large-scale research.

Fast Response

NGS has a short turnaround time, from sample preparation to data generation in just a few days, improving research efficiency. Tsingke ensures that clients have timely access to data, providing fast and accurate services.

Bio-credentials Analysis Team

Tsingke BioSignal analysis team has rich experience in multi-omics analysis, ensuring efficient analysis and interpretation of data, and providing comprehensive support from data generation to result analysis.

Direct detection of modified bases

Third-generation sequencing technology can directly detect modified bases in DNA, such as methylation status, without additional processing steps. This provides an important tool for epigenetic studies and contributes to a deeper understanding of gene reg

FAQ

What are the advantages of whole genome sequencing over whole exome sequencing?

Whole-exome sequencing captures and sequences only the exonic regions of the genome, and its genomic information accounts for about 1.5% of the entire genome size, and it can be used for SNP, InDel and CNV analyses, whereas whole-genome sequencing sequences the entire genome, and it can also be used for SV analyses. In addition, whole genome sequencing detects more comprehensive mutation information, not stopping at coding regions, but expanding to the entire non-coding regions. Mutations in non-coding regions have been increasingly studied in recent years and can be associated with the development of complex diseases, including many types of cancer.

For bacterial genome sequencing, what are the advantages of third-generation sequencing over second-generation sequencing?

Compared with second-generation sequencing, third-generation sequencing has the advantage of long reads and low GC content, while the disadvantage of high sequencing cost. For bacterial genome sequencing, the long read length of triple-generation sequencing can solve the problem of repetitive sequences in bacteria and avoid the problem of uneven sequencing of abnormal GC strains. Due to the small size of the bacterial genome, the amount of sequencing required is not large, and the cost of triple-generation is even lower than that of the second-generation combined with the first-generation strategy for the finer bacterial completion maps. So far, at the level of bacterial framework maps that require lower assembly completeness, second-generation sequencing still maintains a certain cost advantage. With the improvement of throughput and cost reduction of three-generation sequencing, it is expected that three-generation sequencing will be more widely used in the field of bacterial genomes in the future.

3. Which species can perform eukaryotic reference transcriptome sequencing?

Eukaryotic reference transcriptome sequencing requires the following species:

a. The species is eukaryotic;

b. The species has a reference genome, spliced at least to the scaffold level;

c. The species has a relatively complete annotation.

Note: If the reference genome is not available, eukaryotic non-reference transcriptome sequencing can be chosen.