Antisense oligonucleotides (ASOs) are short, synthetic, single-stranded oligodeoxynucleotides that typically contain 15 to 25 monomers. They are designed to specifically target mRNA and inhibit its translation into proteins.
Upon cellular entry, ASOs can trigger mRNA degradation by specifically binding to complementary target sequences, facilitated by ribonuclease H1 (RNase H1) activity, consequently suppressing protein expression. Alternatively, ASOs can modulate gene translation by exerting steric hindrance effects on pre-mRNA, thereby achieving selective splicing and contributing to therapeutic interventions for various diseases.
Tsingke provides comprehensive ASO synthesis services with strict adherence to ISO 13485:2016 quality standards to ensure the delivery of high-quality products. We offer a wide range of modifications to enhance oligo stability, as well as flexibility in synthesis scale from micrograms to kilograms. Our purification options, including ESI mass spectrometry and HPLC, are designed to meet your specific experimental requirements.
| Product |
Modification |
Purification |
QC |
Deliverable |
|
Custom ASO Synthesis |
LNA, cEt, |
RNase free HPLC |
MS optional: HPLC, SEC-HPLC |
· Tube or customized lyophilized RNA · COA report (electronic) |
|
Stage |
Support Provided |
|
Preclinical Research |
- Provide products for pharmacological and toxicological studies - Assist in completing pharmacological and toxicological research - Ensure the safety and efficacy of candidate drugs in animal models |
|
Phase Ⅰ |
-Assist in completing pharmaceutical trials and initial studies - Write submission materials - Provide IND application services |
|
Phase Ⅱ |
- Help determine the efficacy and dose-response relationships of new drugs |
|
Phase Ⅲ |
- Assist in completing pharmaceutical research - Prepare for new drug market entry - Offer documentation writing and submission services |
|
Commercialization |
- Provide support for commercial production - Ensure stable and efficient market supply - Adhere to national and international regulations and standards throughout the drug development process |
