Name: Plasmid Preparation
Brand: Tsingke Biotech
SKU: 00283
Availability: InStock
Rating: 5 (1 reviews)

Plasmid Preparation

Overview

Plasmids are small, circular DNA molecules within cells, commonly used as vectors for DNA recombination. In molecular biology experiments, the target gene is typically inserted into the multiple cloning sites of a plasmid, creating a new recombinant plasmid. The target gene can then enter recipient cells along with the plasmid vector. Utilizing components on the plasmid, replication, and expression can occur within the recipient cells, transferring to progeny cells with host cell division. The quantity and purity of extracted plasmids may vary for different downstream experiments. Obtaining high-quality plasmids is crucial for the success of subsequent experiments.

Research Grade: Obtain increased quantities of plasmid (default concentration 1000 ng/μL);

Transfection Grade: Removes endotoxin, increases supercoil ratio, achieves highly efficient cell transfection.

Advantages

One-stop Solutions

Tsingke provides a comprehensive service encompassing gene synthesis, vector construction, and plasmid preparation

Flexible Customized

Microgram to gram scale quantity

High Industrial Standard

Maximal transfection efficiency with high supercoil content and low endotoxin level

Service Details

Research Grade
Volume	*Turnaround Time(Business Day)	Deliverables
100 μg	5	Prepared lyophilized plasmid DNA; Sequencing map (.abl file); COA Report(electronic).
200 μg	5
500 μg	7
1 mg	7
2 mg	8
3 mg	9
4 mg	10
5 mg	11
10 mg	13
20 mg	13
50 mg	15
100 mg	18

*Opt for Gene Synthesis Alongside Plasmid Preparation and Save 2 Business Days on Turnaround Time

Get your quote by emailing gene@tsingke.com.cn

Quality Control
Research Grade	Items	Method	Specifications
	Appearance	Visualinspection	Colorless Liquid of Pure Transparency
	A260/280 ratio	UV Absorbance	1.8~2.0
	Supercoil content	Agarose gel electrophoresis	＞50%
	Concentration	UV Absorbance	90%~110%
	Residual DNA	Agarose gel electrophoresis	Not visible
	Residual RNA	Agarose gel electrophoresis	Not visible
	Restriction enzyme analysis	Agarose gel electrophoresis	Adjustable according to requirement
	Sequence verification	Sanger sequencing	Consistent with the confirmed plasmid sequence

Transfection Grade
Endotoxin	Volume	*Turnaround Time(Business Day)	Deliverables
＜0.1 Eu/ μg	100 μg	5	Prepared lyophilized plasmid DNA; Sequencing map (.abl file); COA Report(electronic).
	200 μg	5
	500 μg	7
＜0.01 Eu/ μg	1 mg	7
	2 mg	8
	3 mg	9
	4 mg	10
	5 mg	11
＜0.005 Eu/ μg	10 mg	13
	20 mg	15
	50 mg	17
	100 mg	19

*Opt for Gene Synthesis Alongside Plasmid Preparation and Save 2 Business Days on Turnaround Time

Get your quote by emailing gene@tsingke.com.cn

Quality Control
Transfection Grade	Items	Method	Specifications
	Appearance	Visual inspection	Colorless Liquid of Pure Transparency
	A260/280 ratio	UV Absorbance	1.8~2.0
	Supercoil content	Agarose gel electrophoresis	＞85%
	Concentration	UV Absorbance	90%~110%
	Residual DNA	Agarose gel electrophoresis	Not visible
	Residual RNA	Agarose gel electrophoresis	Not visible
	Restriction enzyme analysis	Agarose gel electrophoresis	Adjustable according to requirement
	Sequence verification	Sanger sequencing	Consistent with the confirmed plasmid sequence
	Endotoxin	Limulus amebocyte lysate (LAL) test	Endotoxin ≤ Standard
	Exogenous Contamination Detection	NGS(Depth>30×)	Genomic DNA < 1% Other DNA Contamination < 0.1%

Workflow

Transfection Grade

Figure1.Optical density measurement software display diagram

FAQ

What do I need to provide for Plasmid Preparation service?

We provide the following two options:
a. Gene synthesis + plasmid preparation: Synthesize your gene by Tsinkge our and then proceed with plasmid preparation.
b. Plasmid preparation only: You need to ship the target plasmid to us.
Download the "Tsingke Gene Synthesis Order Form" from our website, fill in the relevant fields according to your needs, and send it via email to gene@tsingke.com.cn to place your order.

Can the obtained plasmids be used for cell transfection?

Yes, Tsingke provides transfection-grade plasmid preparation services with low endotoxin levels and high supercoiled DNA content, suitable for cell transfection, gene therapy, and gene editing.
Endotoxin levels can be selected as ≤0.1 EU/μg, <0.01 EU/μg, or <0.005 EU/μg.

**For Research Use Only. Not for use in diagnostic procedures.

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