DNA Fragments

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Overview

DNA fragments are short segments of DNA molecules, typically ranging from a few base pairs to several thousand base pairs. They are crucial in molecular biology for various applications such as polymerase chain reaction (PCR) and DNA sequencing technology.

Tsingke's fragment service provides double-stranded DNA fragments, synthesizing lengths of up to 1,200 base pairs, complemented by Sanger sequencing validation. 

This service caters to the requirements of straightforward gene construction or modification, empowering you to accelerate your research and development activities for various applications such as antibody development, CRISPR-mediated genome editing, and NGS controls. Our DNA fragment synthesis service empowers you to drive innovation and progress in your projects.

 

Advantages
Tsingke’s DNA fragment synthesis service offers high-quality DNA fragments up to 1,200 base pairs. With free codon optimization, a low error rate through column-based synthesis, and optional Sanger sequencing validation, researchers can streamline DNA fragment synthesis and gene block construction with confidence. This fragment synthesis service provides a reliable and efficient solution, accelerating R&D processes, , making it ideal for gene fragment synthesis applications like antibody development, CRISPR genome editing, and NGS controls.
Free
Free Codon Optimization
Low Error Rate
Column-based synthesis
Optional
Sanger sequencing validation
Service Details
Tsingke’s DNA fragment synthesis service provides double-stranded DNA fragments with lengths ranging from 100 bp to 1.2 kb, delivering lyophilized DNA with a fast turnaround time of 5-7 business days. This service ensures high-quality gene fragment synthesis with optional Sanger sequencing validation for added accuracy. The application range includes gene cloning, molecular labeling, gene mutation research, molecular diagnosis, and genome editing, making it a versatile solution for gene block construction and fragment synthesis in various research and development projects.

Length

Turnaround time (Business Day)

Deliverables

100 bp~1.2 kb

2~3

1 tube of lyophilized DNA
 (about 500 ng/ tube); 

COA Report (electronic). 

Workflow
sequence analysis & codon optimization
Sequence Optimization
sequence analysis & codon optimization
design & synthesis
Oligo Synthesis
design & synthesis
overlap PCR
Fragments Assembly
overlap PCR
ship the gene fragments
Delivery
ship the gene fragments
Related Resource
FAQ
What deliverables are provided for DNA fragment synthesis?
A tube containing approximately 500 ng of lyophilized DNA, Electronic Certificate of Analysis (COA), Sequencing Chromatograms (.ab1 files) and Target Sequences (.seq files) if sequencing validation is needed.
**For Research Use Only. Not for use in diagnostic procedures.
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