Name: Custom RNA Oligos
Brand: Tsingke Biotech
SKU: 00303
Availability: InStock
Rating: 5 (1 reviews)

Advantages

Our custom RNA oligos encompass a wide range of synthesis types, including sgRNA, siRNA, and miRNA. As a leading RNA synthesis company, we deliver high-quality, cost-effective RNA oligos with rapid turnaround times—most orders are completed within just 3-4 days. We prioritize quality through 100% mass spectrometry, with additional QC methods available to meet your specific needs. No matter which type of RNA oligos you require, our custom rna oligo synthesis service is committed to delivering excellence.

Various Product Types

Various RNA synthesis products, such as sgRNA, siRNA, miRNA, etc.

Rapid Synthesis Cycle

Regular orders can be completed in 3-4 days

Various QC Methods

100% mass spectrometry with a variety of additional detection options

Service Details

Our Custom RNA Oligo Synthesis Service offers a high-quality, cost-effective solution for researchers needing precise and reliable RNA oligos. Whether you require sgRNA, crRNA, siRNA, miRNA or ASO, our service could meet your require, with options for custom modifications and high-level purification like OPC and HPLC etc. With quick turnaround times and rigorous quality control, including mass spectrometry etc., we ensure every custom RNA oligos meets the highest standards. Order RNA oligos tailored to your research needs from a trusted RNA synthesis company, and experience the convenience and excellence of our custom RNA synthesis service.

Name	Type	Quantity	Purification	Turnaround Time (Bussiness Day)	QC	Modification
sgRNA	sgRNA	1.5 nmol	OPC	7	Mass Spectrometry	Add specific modifications
sgRNA	sgRNA	1.5 nmol	HPLC	12~15	Mass Spectrometry HPLC
crRNA	crRNA	5 nmol /2 OD	HPLC	12~15	Mass Spectrometry HPLC
siRNA Oligo Duplex	1 siRNA	5 nmol /2 OD	HPLC	3~4	Mass Spectrometry HPLC	/
	siRNA NC	2.5 nmol /1 OD		Available in stock		/
	3 siRNA, 1 Guaranteed	5 nmol /2 OD		3~4		3 unique siRNA duplexes/ FAM labeled NC (1 nmol)/ Negative and Positive control (2.5 nmol each) At least one guaranteed gene knockdown (≥70%)
	4 siRNA, 1 Guaranteed	5 nmol /2 OD		3~4		4 unique siRNA duplexes/ 1 FAM labeled NC (1 nmol)/ Negative and Positive control (2.6 nmol each) At least one guaranteed gene knockdown (≥70%)
miRNA	miRNA mimics	5 nmol/2 OD	HPLC	3~4	Mass Spectrometry HPLC	/
	miRNA inhibitor	10 nmol/2 OD		3~4
	mimics/ inhibitor(NC)	1 OD		Available in stock
	miRNA agomir	2 OD		5~7
	miRNA antagomir	2 OD
	agomir/ antagomir (NC)	2 OD
ASO	PS-ASO	2 OD	HPLC	3~4	Mass Spectrometry HPLC	/
	LNA-ASO	2 OD
	OMe-ASO	2 OD

FAQ

What is the maximum length and the types of RNA oligos synthesized by Tsingke?

We specialize in synthesizing RNA oligos up to 180 nucleotides in length, and the types mainly include siRNA, sgRNA, miRNA, ASO, and various modified RNAs.

What are the maximum absorption and emission wavelengths of commonly used RNA fluorescent dyes?

FAM has a maximum absorption at 495 nm and maximum emission at 520 nm. Cy3 has a maximum absorption at 550 nm and maximum emission at 565 nm. Cy5 has a maximum absorption at 643 nm and maximum emission at 667 nm.

How should siRNA be dissolved and stored?

Before opening, centrifuge the tube. Add sterile RNase-free H2O or ddH2O to prepare a 20 μM stock solution. Aliquot as needed and store at -20°C to -80°C.

Why is a positive control important in RNA interference experiments?

A positive control is crucial for validating the experimental system. When the expected results from the siRNA positive control are observed, it ensures that the transfection, RNA extraction, and QC methods used are reliable.

What roles do positive and negative controls play in RNAi experiments, and how should I select?

Positive controls are verified siRNAs targeting housekeeping or reporter genes, used to assess the feasibility of the experimental methods. Tsingke provides effective siRNA positive controls targeting GAPDH, ACTB, GFP/EGFP.

Negative controls are non-specific siRNAs used to demonstrate the specificity of siRNA effects and can be selected from universal or scrambled sequences based on requirements.

Should the results of negative controls be the same across different groups? What if there is significant deviation?

Normally, the results of negative controls should be similar across different groups. Significant deviations may indicate issues with the accuracy of experimental results.

For certain genes, particularly those related to cellular stress or immune response, varying responses to external stress might cause inconsistent gene expression across control groups.

What should I do if the silencing effect is not satisfactory?

The two most common reasons for unsatisfactory silencing effects are low transfection efficiency and suboptimal siRNA sequence design.

1.Check Transfection Efficiency: If you are using siRNA for the first time or working with a new cell line, we recommend testing and optimizing the transfection efficiency. If you have already optimized the transfection conditions and the problem persists, consider switching to a different transfection reagent or method, as this might improve transfection efficiency.

2.Evaluate siRNA Sequence Design: If the transfection efficiency has been improved but the silencing effect is still not satisfactory, it may be due to ineffective siRNA sequence design.