Custom RNA Oligos

Poor cell status, need to adjust cell growth status: generally cells in good growth status have better tolerance to transfection reagents; siRNA or transfection reagent concentration is too high; transfection reagent is more toxic, replace transfection reagent; siRNA purity is low, impurities affect cell status; transfection time is too long, not timely replacement of fresh medium after transfection.

siRNA purity is too low, pay attention to lysis, avoid RNA enzyme contamination, use consumables and reagents treated by DEPC; siRNA transfection reagent complex concentration is low, need to optimize transfection efficiency, use appropriate transfection reagent dose; serum affects transfection efficiency, when preparing the mixture, it should not contain serum; culture can have serum, but do not have Antibiotics; 

The product is shipped in the form of lyophilized powder, the dry powder is attached to the wall of the tube and is easily dispersed when opened, so please centrifuge the tube for a few seconds before dilution to make the product gather at the bottom of the tube and open the cap carefully, cover the tube after dissolution, vortex and shake to mix to make full dissolution. It is recommended to store at -20 ℃ or below, avoid repeated freezing and thawing, and keep in separate containers. Centrifuge instantly before use and prepare 20 μm-100 μm storage solution with RNase-free Water or ddH2O, the average molecular weight of siRNA is 13300. RNA handling rules should be strictly followed and RNase-free lab supplies should be used for experimental operations to avoid degradation of RNA. Please keep on ice when using this product. For RNA with fluorescent marker, we use brown centrifuge tube to pack, because the fluorescent marker is sensitive to light, it must be stored away from light.