Recent research highlights the effectiveness of single-stranded DNA (ssDNA) as a homology-directed repair donor template in CRISPR gene editing. Compared to double-stranded DNA (dsDNA), ssDNA significantly boosts editing efficiency, reduces off-target effects. Its versatility extends to various biological reactions, particularly in DNA nanotechnology applications.
In CRISPR and CRISPR-Cas9 genome editing experiments, long ssDNA serves as a potent donor template, enhancing both insertion and gene replacement efficiency. Its utility extends to single-strand conformation polymorphism, in vitro transcripti studies, nucleic acid enzyme S1 mapping, probe preparation, labeling, and differential hybridization.
Beyond laboratory experiments, ssDNA plays a role in DNA nanotechnology, where it serves as a scaffold for drug delivery, molecular diagnostics, DNA-based data storage, and diverse nanoscale applications. With a lower risk of random integration, ssDNA is particularly suitable for gene editing in primary cells, stem cells, and the creation of genetically modified animal models.
To support these advancements, Tsingke now offers high-quality, sequence-validated ssDNA to optimize the efficiency of CRISPR experiments. Our ssDNA synthesis service ensures reliability and accuracy, empowering researchers in their gene editing endeavors.
